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Organoids and Engineered Organ Systems - 2018

1 Results

Original Research
Open Access
A High-Throughput Organoid Microinjection Platform to Study Gastrointestinal Microbiota and Luminal Physiology
Cellular and Molecular Gastroenterology and Hepatology
Vol. 6Issue 3p301–319Published online: May 21, 2018
- Ian A. Williamson
- Jason W. Arnold
- Leigh Ann Samsa
- Liam Gaynor
- Matthew DiSalvo
- Jordan L. Cocchiaro
- and others
Cited in Scopus: 128
- Video
The human gut microbiota is becoming increasingly recognized as a key factor in homeostasis and disease. The lack of physiologically relevant in vitro models to investigate host–microbe interactions is considered a substantial bottleneck for microbiota research. Organoids represent an attractive model system because they are derived from primary tissues and embody key properties of the native gut lumen; however, access to the organoid lumen for experimental perturbation is challenging. Here, we report the development and validation of a high-throughput organoid microinjection system for cargo delivery to the organoid lumen and high-content sampling.