Background and Aims
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Ethical approval and consent to participate
The use of clinical specimens was approved by the institutional review board of the University of Hong Kong and the Hong Kong Hospital Authority. Animal experiments were performed with the approval of Committee on the Use of Live Animals in Teaching and Research (CULATR) of the University of Hong Kong. All experimental procedures were strictly followed to the animals (Control of Experiments) ordinance of Hong Kong.
Consent for publication
All authors have agreed to publish this manuscript. The content of this manuscript is not under consideration for publication elsewhere.
Availability of supporting data
The authors confirmed that the data supporting the findings of the study are available in the article and supplementary materials. Supplementary tables are provided as Excel tables. The genome-wide transcriptomic sequencing and chromatin isolation by RNA purification sequencing are available in the SRA database: PRJNA786081. This paper does not generate original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.
The authors declare no conflicts of interest.
This study was supported by RGC-TBRS (T12-704/16-R) and HKU seed fund for translational and Applied Research (201910160019). Irene OL Ng is Loke Yew Professor in Pathology.
We thank the Centre for PanorOmic Sciences of LKS Faculty of Medicine for their technical support. We also thank the Laboratory Animal Unit of the University of Hong Kong for the animal holding.
Lok-Sze Wong and Lai Wei contributed equally to this work.
The State Key Laboratory of Liver Research, Department of Pathology, Li-Ka Shing Faculty of Medicine, The University of Hong Kong
C.M.W., L.S.W, and L.W. designed the experiments. L.S.W., L.W., H.C.T., C.T.L., G.C.W., and W.S.C. performed the experiments. C.M.W., L.S.W., L.W. analyzed the data. C.M.W. and L.S.W. wrote the manuscript. C.M.W and I.O.N. supervised the study.
Correspondence to Dr Chun-Ming Wong
Our integrated transcriptomic data and functional CRISPR-based screening reveal functional long noncoding RNAs that are frequently overexpressed in HCC. CASC11, the top candidate in the library screening, is shown to regulate the expression of MYC in a cis-regulatory manner, which consequently dysregulates a subet of cell cycle regulators and drives HCC tumor growth.
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